Pre Lab 3 - the spectrophotometer ] to monitor the enzyme...

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Laboratory 3 Preworksheet (BIO130) Each of the following questions is worth 0.5 points each. 1. (Circle the best answers) Generally, the enzyme [ is / is not ] changed by the process, and [ can / cannot ] bind to another target molecule to repeat the process. 2. In enzyme action, the substrate is converted into a Product and then released from the _ Enzyme . 3. 5 ml is equal to a) .05 μ l b) .05 L c) .005 L d) 50 μ l e) None of the above 4. A P-200 is accurate for measuring a) 25 μ l b) 180 ml c) 18 ml d) 0.1 ml e) b and d f) a and d 5. (Circle the best answers) The darker the solution, the [ more / less ] light can travel through the spectrophotometer. This is a measure of [ transmittance / absorbance ]. 6. (Circle the best answers) In the qualitative portion of today’s lab, we use our [ eyes / the spectrophotometer ] to monitor the enzyme activity. In the quantitative portion of the lab we use our [eyes /
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Unformatted text preview: the spectrophotometer ] to monitor the enzyme activity. 7. We test for peroxidase in celery and carrots today. The purpose of this experiment is 1 to demonstrate a) that plants have peroxide b) that peroxidase is found in all plant cells c) that peroxidase is localized to specific cells in a plant d) that peroxidase has a Vmax. 8. (Circle the best answer) If you had much [ more / less ] enzyme than substrate, the reaction might never reach Vmax. 9. You should a) always change pipette tips after every use. b) rinse pipette tips with sterile water between uses. c) leave the lids open on the sterile pipette tip boxes while you are working. d) leave pipette tips on the micropipetter until you are ready to use them. 10. __ Tyrosinase _ converts L-Dopa to dopachrome 2...
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Pre Lab 3 - the spectrophotometer ] to monitor the enzyme...

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