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exp+15+16+sum08

exp+15+16+sum08 - #12 Endospore stain Green endospores Red...

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#12 Endospore stain Green- endospores Red- vegetative cells Bacillus megaterium
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RESULTS acid-fast bacteria non acid-fast bacteria #13 Acid-fast stain Mycobacterium smegmatis Escherichia coli
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Use of micropipettors (p. 72)- 3 types: 2/20 yellow button. Measures 2-20 μ l 20/200 gold button. Measures 20-200 μ l 100/1000 blue button. Measures 200-1000 μ l Always use new sterile tip for each transfer. Discard tips in vesphene beaker 1000 μ l = 1 ml 100 μ l= 0.1 ml
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Serial dilutions (p. 73) To count number of microbes present in a sample, must dilute it several times to get a countable number of cells. Dilution = volume transferred volume transferred + volume of blank __1 ___ = __1 ___ 1 + 99 100
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Bacterial numbers are so high that a series of these dilutions is necessary to achieve a countable (30-300; statistically significant) plate. Purpose- determine number of colony forming units per milliliter (cfu/ml) in a sample. cfu/ml in original culture # of colonies on countable plate reciprocal of the dilution factor of the countable plate X = Example: (52) 100 1 = 5200 = 5.2 x 10 3 cfu/ml
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SERIAL DILUTIONS OBJECTIVE: To determine the no. of colony forming units/ml in the original culture. diluting the broth culture to obtain a countable plate countable plate is 30-300 colonies #of colonies on x reciprocal of the dilution factor countable plate on the countable plate 159 x 10 3 = 1.59 x 10 5 pp. 73-77
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Dilution = volume transferred .
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