Exp+12+and+13+sum08

Exp+12+and+13+sum08 - Exp#12 Endospore stain Endospore...

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Exp #12 Endospore stain Endospore - dormant survival structure formed by some Gram positive rods when conditions are unfavorable Endospores are extremely resistant to heat, dehydration, chemicals, etc. They can survive indefinitely. Differential stain
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Sporulation – process of spore formation Vegetative cell – a cell that can grow and divide under optimal conditions Germination – cell becomes hydrated, emerges from the spore coat, and returns to a vegetative cell Endospore – a survival structure in a cryptobiotic state; contains dipicolinic acid complexed with calcium
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G + rods that form endospores Clostridium C. botulinum botulism C. tetani tetanus C. perfringens gas gangrene Bacillus B. anthracis anthrax
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Primary Stain - Malachite green “Mordant” – heat from steam drives stain into cells Decolorizer - water to remove stain from vegetative cells Counterstain - safranin Results Pink/Red vegetative cells Green endospores Shaeffer Fulton Endospore Stain
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Endospore staining Procedure – pg 55 1. 1 smear/person of either Bacillus megaterium or Bacillus sphaericus
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Exp+12+and+13+sum08 - Exp#12 Endospore stain Endospore...

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