Exp+11+sum08

Exp+11+sum08 - ability to retain the primary stain and...

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Simple stain photos- exp 7,8 Page 35
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Streptococcus mutans streptococcus
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Staphylococcus epidermidis
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Bacillus subtilis- random bacilli
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Escherichia coli- random bacilli
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Spirosoma linguale- random spirals
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Bacillus megaterium- streptobacilli
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Streptomyces coelicolor- filamentous
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Deinococcus radiodurans- tetrads
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#11 Gram stain Differential stain Difference between simple and differential stains? Staining differences between Gram + and Gram - are related to cell wall structural differences.
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#11 Gram stain Spirosoma is G- Staphylococcus is G+
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Gram + have a thick peptidoglycan layer. Alcohol causes pores in the walls to close, preventing the escape of insoluble crystal violet-iodine complex. Gram – bacteria: alcohol penetrates lipopolysaccharide layer and the thin peptidoglycan layer does not prevent the escape of the crystal violet-iodine complex. Gram reaction is most valid on young cultures (24 hours or less). Some Gram + organisms lose their
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Unformatted text preview: ability to retain the primary stain and appear Gram - (pink). 72 hour culture Gram standard- photo pg 107 Spirosoma -Gram -, spirals Staphylococcus -Gram +, cocci PROCEDURE - pg. 2 smears/person a. Gram standard b. Pure culture plate 1) 1 loopful of Gram standard. ( Use aseptic technique Pg ) 2) Make THIN SMEARS 3) Air Dry/Heat-fix. 4) Allow slide to cool, cover with crystal violet 1 min. 5) Rinse with water. Cover with Grams iodine 1 min. 6) Rinse with water. Briefly drop ethanol 7) Rinse with water 8) Cover with safranin 1 min. 9) Rinse with water. Blot with bibulous paper 10) Observe under oil immersion. 11) Record observations on pg. ) 1 smear/person from Gram standard 48 49 Each person does 1 Gram stain: Gram standard is a mixture of Spirosoma linguale Staphylococcus aureus Complete page 49 chart...
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This note was uploaded on 10/01/2008 for the course BIOL 2051 taught by Professor Brininstool during the Summer '07 term at LSU.

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Exp+11+sum08 - ability to retain the primary stain and...

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