{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Exp+11+sum08 - ability to retain the primary stain and...

Info icon This preview shows pages 1–17. Sign up to view the full content.

View Full Document Right Arrow Icon
Simple stain photos- exp 7,8 Page 35
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Streptococcus mutans streptococcus
Image of page 2
Staphylococcus epidermidis
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Bacillus subtilis- random bacilli
Image of page 4
Escherichia coli- random bacilli
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Spirosoma linguale- random spirals
Image of page 6
Bacillus megaterium- streptobacilli
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Streptomyces coelicolor- filamentous
Image of page 8
Deinococcus radiodurans- tetrads
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
#11 Gram stain Differential stain Difference between simple and differential stains? Staining differences between Gram + and Gram - are related to cell wall structural differences.
Image of page 10
#11 Gram stain Spirosoma is G- Staphylococcus is G+
Image of page 11

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Gram + have a thick peptidoglycan layer. Alcohol causes pores in the walls to close, preventing the escape of insoluble crystal violet-iodine complex. Gram – bacteria: alcohol penetrates lipopolysaccharide layer and the thin peptidoglycan layer does not prevent the escape of the crystal violet-iodine complex. Gram reaction is most valid on young cultures (24 hours or less). Some Gram + organisms lose their
Image of page 12
Image of page 13

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 14
Image of page 15

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 16
Image of page 17
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ability to retain the primary stain and appear Gram - (pink). 72 hour culture Gram standard- photo pg 107 Spirosoma -Gram -, spirals Staphylococcus -Gram +, cocci PROCEDURE - pg. 2 smears/person a. Gram standard b. Pure culture plate 1) 1 loopful of Gram standard. ( Use aseptic technique – Pg ) 2) Make THIN SMEARS 3) Air Dry/Heat-fix. 4) Allow slide to cool, cover with crystal violet – 1 min. 5) Rinse with water. Cover with Gram’s iodine – 1 min. 6) Rinse with water. Briefly drop ethanol 7) Rinse with water 8) Cover with safranin – 1 min. 9) Rinse with water. Blot with bibulous paper 10) Observe under oil immersion. 11) Record observations on pg. ) 1 smear/person from “Gram standard” 48 49 • Each person does 1 Gram stain: – “Gram standard” is a mixture of • Spirosoma linguale • Staphylococcus aureus – Complete page 49 chart...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern