EXPERIMENT_II3 - EXPERIMENT II: BACTERIAL GROWTH AND...

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EXPERIMENT II: BACTERIAL GROWTH AND INDUCTION OF BACTERIAL OPERONS, BACTERIAL GENETICS Clara E. Fletcher MMG 408: Advanced Microbiology Lab November 1 st 2006
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TABLE OF CONTENTS
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INTRODUCTION Part I. Exponential Growth and Induction of the E. coli lac Operon All organisms reproduce in order to pass their genes on from one generation to the next. Bacterium follow this model by performing binary fission, which means that one single cell divides to form two daughter cells, each containing the same genetic information as the parent cell. Bacteria will replicate as often as they possibly can, or more often in nature, as often as their external cellular environment allows, so their growth is exponential, and can be represented linearly. In this experiment two methods were used to measure bacterial growth, optical density (O.D.) measurements as well as viable cell count measurements, achieved from plating the bacteria mixtures prepared and measuring titers. In nature, often the external environment varies, so bacteria can conserve energy
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This note was uploaded on 03/18/2008 for the course MMG 408 taught by Professor Arvidson during the Spring '08 term at Michigan State University.

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EXPERIMENT_II3 - EXPERIMENT II: BACTERIAL GROWTH AND...

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