EXPERIMENT_IV - EXPERIMENT IV: ISOLATION AND...

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EXPERIMENT IV: ISOLATION AND CHARACTERIZATION OF HERBICIDE DEGRADING BACTERIA Mark Gaynor MMG 408 12/13/2007
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Introduction Bacteria are able to metabolize an enormous amount of different compounds for energy, due both to their physiological and genealogical adaptations. These adaptations can be very useful for a variety of purposes, including dissimilatory metal reduction, soil enhancement, and for this experiment, biodegradation. Microbes are capable of breaking down many environmental toxins, using their carbon as an energy source for their own metabolism. The organic toxin used in this experiment was 2,4 Dichlorophenoxyacetic Acid (2,4-D), an herbicide. By growing a culture in a medium containing 2,4-D, microbes can be enriched to grow in a medium with the toxin as the sole carbon source, therefore also enriching the breakdown of 2,4-D in the medium. The ability to utilize a number of compounds as energy sources and to survive many different types of environments takes constant evolution, and microbes are set up to evolve their DNA sequences through horizontal gene transfer. Bacteria are able to easily accept and exchange genes from and with their environment, through processes of conjugation, transduction, and plasmid and transposon integration. Another type of genetic sharing involves triparental mating. Triparental mating involves three different strains of cells: the selected recipient strain, a strain with a mobilizable plasmid, and a strain containing a self-transmissible plasmid. The mobilizable plasmid, p1062a, contains the Tn5 transposon, containing the desirable trait of kanamycin resistance. This plasmid is not capable of transferring itself, but it can be transferred by another plasmid in the same cell. The self-transmissible plasmid pRK2014 was used in this experiment, and it does mobilize the p1062a plasmid, we can assume the two share an oriT sequence.
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EXPERIMENT_IV - EXPERIMENT IV: ISOLATION AND...

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