EXPERIMENT_IV - EXPERIMENT IV ISOLATION AND...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
EXPERIMENT IV: ISOLATION AND CHARACTERIZATION OF HERBICIDE DEGRADING BACTERIA Mark Gaynor MMG 408 12/13/2007
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Introduction Bacteria are able to metabolize an enormous amount of different compounds for energy, due both to their physiological and genealogical adaptations. These adaptations can be very useful for a variety of purposes, including dissimilatory metal reduction, soil enhancement, and for this experiment, biodegradation. Microbes are capable of breaking down many environmental toxins, using their carbon as an energy source for their own metabolism. The organic toxin used in this experiment was 2,4 Dichlorophenoxyacetic Acid (2,4-D), an herbicide. By growing a culture in a medium containing 2,4-D, microbes can be enriched to grow in a medium with the toxin as the sole carbon source, therefore also enriching the breakdown of 2,4-D in the medium. The ability to utilize a number of compounds as energy sources and to survive many different types of environments takes constant evolution, and microbes are set up to evolve their DNA sequences through horizontal gene transfer. Bacteria are able to easily accept and exchange genes from and with their environment, through processes of conjugation, transduction, and plasmid and transposon integration. Another type of genetic sharing involves triparental mating. Triparental mating involves three different strains of cells: the selected recipient strain, a strain with a mobilizable plasmid, and a strain containing a self-transmissible plasmid. The mobilizable plasmid, p1062a, contains the Tn5 transposon, containing the desirable trait of kanamycin resistance. This plasmid is not capable of transferring itself, but it can be transferred by another plasmid in the same cell. The self-transmissible plasmid pRK2014 was used in this experiment, and it does mobilize the p1062a plasmid, we can assume the two share an oriT sequence.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern