Chapter 3-4 notes - that it would bind specifically Add...

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Purify the following: The mannose binding proteins MBP is an oligomeric protein with a molecular mass of 200 kDa and an isoelectric point of 4.4. How would you get this protein pure? - Size exclusion would be good because 200 kDa is actually pretty big, - Ion exchange that goes for isoelectric point. At pH 7 it would be negatively charged, so we would anion exchange chromatography - But Affinity chromatography would be best! Mannose would be put in the matrix so
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Unformatted text preview: that it would bind specifically. Add more mannose so it will bind to the free mannose and elude out! Prolyl hydroxylase catalyzed yhr hydroxylation of praline to hydroxyproline; cofactor of prolyl hydroxyulase is Fe2+, which easily oxidizes – asorbic acid (Vit V) One of the diseases that affect tenages as well as malnourished elderlies these days due to dietary lack of vit C bleeding gums loose teeth muscle aches....
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