MCB 102 F08 Lect _3 DRAFT

MCB 102 F08 Lect _3 DRAFT - Announcements There are...

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Announcements There are electronic bulletin boards available on the bSpace site for MCB 102 F08 (see "Forums"), where you can post to your classmates questions that you may have about the material in this course, if you so choose. If you have questions that you feel only Prof. Thorner can answer, PLEASE attend his weekly office hours on Tuesdays 2-4 PM and Fridays 1-3 PM (rather than sending your question by e-mail). Assignments to Discussion Sections are permanent, unless you encountered an unforeseen and legitimate conflict with another course in which you are enrolled and which you can demonstrate is required for your major. NO swaps after 5 PM, Fri., 9/5.
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Painting from David Goodsell, The Scripps Institute A Cell Comprises Many Different Types of Molecules The Constellation of Proteins in a Cell is its "Proteome"
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A whole-cell (crude) extract A single pure protein PURIFICATION A complex mixture is resolved or deconvoluted into its constituents by the successive application of physicochemical methods for separation and fractionation Analysis of its AA sequence functional characteristics Determination of its 3D structure Understanding the Function of a Protein Requires its Purification "Don't waste clean thoughts on dirty enzymes." —Arthur Kornberg (1918-2007)
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+ 3 AA2 AA1 AA3 + Tripeptide: A protein is a chain of amino acid residues joined by amide bonds amide bonds an amino acid "residue"
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+ 3 the N-terminal α -amino group the C-terminal ( ϖ ) carboxyl group n Characteristics of the side chains?
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MCB 102 F08 Lect _3 DRAFT - Announcements There are...

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