BIMM 100 - maho midterm

BIMM 100 - maho midterm - Bib/[M100 ~ Midterm First name: 8...

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Unformatted text preview: Bib/[M100 ~ Midterm First name: 8 . Last name: :9 1.1145 pointsl A. Human DNA contains 20% C on a molar basis. What are the molar percentage of A, G and T? (3) A 30°/o G- 209 0 T 30% B. A segment of DNA from the interior of a single strand is shown in Figure 1. What is the polarity pf this DNA from top to bottom? and explain why (3) J a S’dnd —" 5‘ hydrant“ or Phosphaha qrowP on I the S’cubon 0F suqarCPentose) (ribse) O 3C"d‘“" a hydroqu qrowp on the. B’mrbon of 9 - I C. Most of the DNA sequences in a bacterial genorne code for proteins, whereas most of the sequences ; f, in the human genome do not. Is this true or false and explain your answer. (4) True Bacteria. has no ‘cntronic 38%. while. human genome: doesr Figure l D. Write the entire name of dCT P. Describe the major difference between dCT P and ddCI‘ P. What would you expect if ddCT P were added to a DNA replication reaction in large excess over the concentration of dCTP? (6) ~ deoxfl cytosine. 5’ Hipbospkafe. ‘{dd CTP has no ~OH qrowp affearbon 0F sugar-“(ribose/ dCTP "' OH I» I a, a ' -'DNA replication wedd, bolt od‘ 6. BIMMlOO - Midterm ‘ First name: 6/\ Last name: E. Wobble pairing occurs between the first position in the anti-codon and the third position in the codon. Is this correct or false? If false, correct the mistakes. (3) Correct F. Describe steps require energy during translation elongation. (3) ~ * confirmahonafl. Claw-acre. 0F ribosome U—P‘M b'mdcéq To CLGV7€&. {RNA (QQ-tENA) at A sil—e “ - 14:95:: He :1 OJ: .ir..i..§9 30”” ‘3 (-1 i6 .9 “tpeph 6 bond Penis—ahéri' '_ ( EFZ C’rTPm» EFZ (Er-DP) 2. You wish to make a restriction map of a 3.0kb - i 4 E m BamHl restrictionjragment. You digest three - d a: samples of the fragment with'EcoRl , HPAII and Em“ “Pa” Him" a mixture of EcoRI and HpaII. You then separate the fragments by gel electrophoresis and visualize the DNA bands. 1'? kb 1.5 kb From these results, prepare positions of the Wk" 12“) LEcoRl and HpaII recognition sites and the Mk!) 0.9m distances in kilobases (kb) between them. (8) 0.5 kb 0.4 kb 0.4 kh BIMMIOO - Midterm First name: Last name: 3. One strand of a section of DNA isolated from E. coli reads: 5’ GTAGCCTACCCATAGG 3' a. Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? (3) x I 5’ GUAGCCUACCCAUAG'G 3 b. How many different peptides could potentially be made from this sequence of RNA? Would the same peptides be made if the other strand of the DNA served as the template for transcriptiou? (4) 3 d; FFe rent “Fevpb‘dm not 4. What are the four major types of RNA in a cell? What functions do they serve in the cell? (12) cm appropriai‘t’. tRN/Ax "*— iDrihclSAam'lno acid to ribosomes durinq iTMOIai'ion rRNAm” Component 0? fiégagome-F carer.qu Ft}? first-tier“; “RNAW codes For system micffl Known Pumgl'ions include. decrease in Specific. level I, m i'nt'ng'iotl'ioh oF SPELL-Lic- _.__.__ _ ____..__ __._.._.._. _ _ .4 . .__ __ ..______._ farqet_)___.__ BIMMIOO - Midterm First name: Last name: a \ 5. (22 points) a. Describe two features of mRNA which enhance translation. (3) Cap b. Which DNA sequence would be more difficult to denature? And explain why. (4) Q S’AGCTTTACCCGGGGTAAGGS' c/er M ii. 5’ G’ITTAAAACGGCATATAAT 3' 5 iii. 5’ GCGCAATTQAT 3' 6 (i) whim more Grc, rich Se.%m<.e.. Hmfii) Md. lenqer Hum: (iii) TM. TM ‘9? (i) is H16, Highest". c. Describe one critical element that differentiates shuttling vector from other cloning vectors and explain why. (6) x: 11200 0r}j}ns 0P rePiicaHOn For repftlea'fi‘n? (fixt- yeast Mai Emil) 3» fwo clippeth Omidm'i‘ms. d. Describe three different methods used to identify the function of a gene and provide one sentence description of the techniques. (9) * Khocktwtt - De-sruft'ion 0F the creme. QC “Infant in Cmbnfohic. Elem cells 13 Mable k/D mom - RNAL ~— Desrwp‘cmh oF mam man, a. sewn? inferest In! aprecs‘mci miRNA or QiPNA (or antigensia. RNA) ‘ Transgenic. 5 _ — Seqmcz mmvarison (blwlnopneh‘r. acne Fam‘uiu i BIMMIOO - Midterm First name: % v Last name: 6 '6- 5’ A1 Az'Gs C4'G5'Aé T7 Cs‘C9‘C1o A111 C12 3’ (6) i. Which nucleotide has a triphosphate group? A 1 ii. How many phosphodiester bonds are present in the strand shown? a lever] iii. Which triphosphate of the nucleotide formed a phosphodiester bond with a 3’-OH group of C4 ' G5 7. What is Okazaki fragments and describe Okazaki fragments joining process. (Including enzymes carry out each steps.) (8) “04" W; Disunh‘nuom DNA Segments swi‘kceijecfl. 0n fine laqfiinq strand DNA WC] DNA reFliwhcn ' Wulhpflp, RNA Primers are h’iadé be! primate... Complementarer II; the :l-aqqinq shame! DNA 4—7 elonqah‘on OF H.902. primer bola DNA Feb-mm w—-§> i l' mo - i ‘ " : - it»? WWW! The?“ {Mi—7’ F ” "1 “"6 as? 5‘1 We 8. What is orepon? Describe advantage and disadvantage of operon. (6) a . I I . FOKquah.G '““ Set; 3.6 t - )nvv/veciirin {fizfietfiezqwcgfns é; I to” a”? 6/” ferre‘E M ‘1 Pmch‘Onwé ream _ K3. _ t . _ _ -- {1' (disarm. Pfié'flmeei " fla’vmf’w : Mot/Eozprof-m ’If'il/Ofl/cae 1'» fee/area? Ewah'onc J. efefiom I. I f ‘I’ ‘ DI'SaJva/nf'oge: IF tmml‘aF‘onrc) beams, P0596266 faca'mpamy £5 peerém- Specific {mamas aw reverafi H ' "1' ' " {Drip—gulf! W3” “63" 'fosf‘ "fimfi/fdnafi-MQ‘ BIMMIOO — Midterm First name: 6: Last name: 9. Explain role(s) of the following proteins. (9) A. Iopoisomera-se ire-lame. oP lorl‘i'onafi. Sl'réss CM 5‘1 “winds”? 0’? “PM 13”" dw'”? Drill-am B' Taq polymerase DNA PO Mae..- thl: cam wit'hd'md mall, ramp. LIA-eel For PC? mm C. Aminoacyl-tRNA synthetase —_.—. a Qpeolééc, 6W1in acid I t3 Effie mrrmspoanclulj, 10. Describe why you need two selectable marker genes for gene replacement (knockout) construct for mouse ES cells. (3) became. a? homclgom rewmbihah‘én ‘In mQYm’hal‘tfln cells :5 V3“! l0”): HM. needs boHo Pail-We, selec—l'ion marker For/\‘Ihl'cqralx‘on flame. neqalw‘ “e Selcch‘cm marhuémn- For clim‘mal'i n1 random - . . . . . . . ‘ Tel? 11. Your fnend performed a northern blot experiment to exarrune 1f 1ncubation With spec1fic 'n set-l“, a? J three different hormones altered expression of BIMM mRNA. The northern blot was also probed at the same time with actin, which does not change with any hormones. Can you mm ' w - choose which one of the hormones is most effective for increase in expression of BIMM RNA? and explain why. (5) Ybbl canno‘b Choose Spech hormones 3} M Q OC-li n mRNA lava! chamqe s » Mimi band, on fine. lei/el all ach‘n, all three- lwoymone Sean B waffK ...
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This note was uploaded on 10/12/2008 for the course BIMM 100 taught by Professor Pasquinelli during the Spring '06 term at UCSD.

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BIMM 100 - maho midterm - Bib/[M100 ~ Midterm First name: 8...

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