Exercise 4 - 7 Characteristics for Stained Characteristics...

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Jonathan Church JC8975 BIO 205L: Analysis for Exercise 3 1. This graph shows the growth of V. natriegens bacteria over a period of 144 minutes. The bacteria were counted by measuring the optical density with a spectrophotometer. 2. 3. 4. The specific growth rate constant, k, is equal to the slope of the best fit line for exponential growth. In this case, k is equal to 0.0183. When calculated for the exponential growth region I found k to be: lnA = lnA 0 +kt ln(0.485) = ln(0.104) + k(108) k=0.0143 5. t doub = 0.693/k = 0.693/0.0143 = 48.46 minutes calculated when eliminating the lag and stationary points t doub = 0.693/k = 0.693/0.0183 = 37.87 minutes experimentally 6. It took approximately 24 minutes before our V. natriegens culture began its exponential growth.
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Unformatted text preview: 7. Characteristics for Stained Characteristics for Non-stained • Has a cell wall (crystal violet) • Organelles that are difficult to see • Transparent • Small microorganisms • Live organisms when observing behavior • Larger microorganisms with color and contrast 8. OD Measurements Direct Counts • More accurate • More convenient • More time efficient • Saves time, therefore more cost efficient • Reduces strain on eyes from looking through a microscope • Less Error due to human mistakes • More accurate estimate when counting a homogenous mixture • Cheaper to stain and count than to spend money on spectrophotometers • Less error due to dirty cuvettes and dirt reflecting light...
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This note was uploaded on 10/12/2008 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas.

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