Spectra max plate reader- SOFTmax PRO 4.3 software.doc

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Spectra max plate reader – SOFTmax PRO 4.3 LS To set up a template: 1. Open SOFTmax PRO 4.3 LS (icon on desktop) 2. File New 3. Under Plate#1 ( grey ) drop-down menu, click on template button Select wells for standards. Under “Group:” menu, select “Standards”. Wells are highlighted in blue . o Denote labels for each BSA concentration (i.e. A, B, C etc) in “Sample:” box o type in concentration in “Concentration” box Select wells for blanks. Under “Group:” menu, select “Blank”. Wells remain grey . Select wells for unknowns. Under “Group:” menu, select “Unknowns”. Wells are highlighted in pink . o Denote labels for each unknown (i.e. B1 a, C2 a, etc) in “Sample:” box. Select wells for unknown dilutions. o Under “Group:” menu, select “New…” o In Name: box, type “Unknown dilutions” o In column format, select “Unknown [Dilutions]”. The “Sample Descriptor” box should now be checked, and “dilution Factor” should be the descriptor.
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