Experiment 4 - Levke Johanna Deutsch Laboratory Report#4...

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Levke Johanna Deutsch Laboratory Report #4 Experiment IV - TLC of Peppermint and Spearmint Oil- Name: Levke Johanna Deutsch Lab Instructor: Chris Henry Date: 06/19/2008 Due to: 06/26/2008 page 1
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Levke Johanna Deutsch Laboratory Report #4 Results and Discussion: Thin-layer chromatography (TLC) is a fast method used to determine whether a specific compound contains certain standards. The purpose of this experiment was to figure out if (R)-Carvone and/or (1R,2S,5R)-(-)-Menthol are in Peppermint Oil. A concentrated drop of a 1% Peppermint oil, (R)-Carvone and (1R,2S,5R)-(-)-Menthol solution was spotted on a TLC plate and placed in a development chamber filled with a 10% Ethyl Acetate/Hexane solution. The TLC plate was removed and the solvent front marked. Under UV light was one dark purple dot and a few little light purple dots spotted and circled. The dark spot comes from the Carvone and has a R f from 0.40. The light purple spots where seen in every compound (Peppermint, Carvone and Menthol) at a R f = 0.22. One more light spot was seen on the Peppermint line. The R
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