column chromatography lab report - Golden 1 Hannah Golden...

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Golden 1 Hannah Golden 18 March 2008 Flourenone Synthesis and Column Chromatography Introduction Chromatography, simply, is the separation of components from a mixture. There are many different types of chromatography including paper, liquid, gas, thin-layer, and for the use of this experiment, column chromatography. Column chromatography is a type of component separation that can be used in large and small scales as a purification technique. In column chromatography, a stationary phase (polar solid, in this case being Alumina), is placed in a glass column and a mobile phase (less polar liquid, in this case hexanes), is added to the top and flows down through the column (by either gravity or flash chromatography). Flash chromatography, used in this experiment, is a solvent force by air pressure. With the polar stationary phase and the less polar mobile phase, the components of a mixture can be separated according to their polarity. It will be seen that the less polar components elute first, leaving the more polar components contained by the stationary phase longer. This method is comparable to TLC chromatography in terms of its mobile and stationary phases and polarities. TLC seems to be more advantageous because of its ability to perform multiple analyses concurrently and it is an easy and fast way of obtaining results. For this experiment, the column and TLC chromatography will be done on the reaction of Fluorene to Flourenone. Fluorene is oxidized under basic conditions and then deprotonated, forming a hydroperoxide. This, in turn forms Flourenone. This can be
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Golden 2 seen by the diagram and electron-pushing mechanism shown below. The diagram is the products, reagents and reactants diagram, showing the beginning and end of the
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This note was uploaded on 03/18/2008 for the course CHEM 213 taught by Professor Masters,katherinelorenzo,peterbisch,amberwilcox,jenniferlchen,ying during the Spring '07 term at Pennsylvania State University, University Park.

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column chromatography lab report - Golden 1 Hannah Golden...

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