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Lab 20 Notes & Prelab

Lab 20 Notes & Prelab - Sarah Song BIO 205.33 Lab 20...

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Sarah Song March 25, 2008 BIO 205.33 Lab 20 Bacterial Transformation Notes Lab Introduction Gene cloning: technique for making copies of desirable genes o Isolate a gene from one organism and transfer to another Desirable genes inserted into plasmids Transformed cell: modified DNA inserted into host cell Antibiotic resistance separated transformed cells from normal cells Plasmids are replicated rapidly o Many copies of inserted genes produced in transformed bacterial cell High level of expression, host bacteria produces large amounts of foreign protein Introduction to Genetic Transformation Genetic transformation: insertion of a gene into an organism in order to change the organism’s traits Genetic transformation in biotechnology: o Agriculture: frost, pest, or spoilage resistance in plants o Bio-remediation: bacteria able to digest oil spills o Medicine: sick person’s cells transformed with healthy copies of the diseased gene Plasmid: small, circular pieces of DNA that enter the bacterial cytoplasm o Usually contain genes for traits beneficial to bacterial survival o Bacteria can transfer plasmids back and forth to share beneficial genes Resistance to antibiotics Genetic engineering: genes that code for new traits can be inserted into a plasmid pGLO plasmid o Genetic features: GFP Green Flourescent Protein Glow in the dark gene taken from the bioluminescent jellyfish Aequorea vitoria Stimulated by pathways specific to jellyfish o E. coli will not be able to glow on their own o Shine UV light on GFP to see emission of green color Ability to resist the antibiotic ampicillin (antibiotic) “DNA switch” Gene regulation system used to control the expression of GFP pBAD and araC promoter
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Sarah Song March 25, 2008 BIO 205.33 Lab 20 Bacterial Transformation o Enables transcription of any gene it is attached to, if arabinose
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