practice midterm

practice midterm - BIMM 100 Midterm Exam Name Please write...

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BIMM 100 Name Midterm Exam Please write your name on each page and look through the entire exam before beginning your answers. Your exam should have 9 * pages, with a total of 8 questions. Please do not use pencil or white-out on your exam. Be certain that all of the work you want graded is written in permanent ink. Note that all sequences are presented in 5' -> 3' orientation and that a codon table and a restriction enzyme table have been provided as the last page of your exam. * This practice exam has only 8 pages because I have not included the codon or enzyme tables from the MCB text. See p 128 or p.177 for these. Your score 1. 16 pts. 2. 24 pts. 3. 15 pts. 4. 9 pts. 5. 8 pts. 6. 12 pts. 7. 18 pts. 8. 18 pts. . Total 120 pts .
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2 Name 1. (4 pts. each/ 16 points total) Circle the number corresponding to the appropriate term/phrase to answer the question or complete the sentence. A. Which anticodon in tRNA recognizes the start codon in translation? 1. UAC 2. AUG 3. GUA 4. CAU 5. UGA B. Which of the following is a protein that is required for translation? 1. peptidyltransferase 2. RNA polymerase 3. ribosomal RNA 4. σ 70 5. aminoacyl-tRNA synthetase C. Which of the following is not needed by bacterial RNA polymerase? 1. a promoter sequence 2. an RNA primer 3. CTP and UTP 4. a DNA template 5. 3’-hydroxyl on ribose D. To amplify DNA using the polymerase chain reaction (PCR), it is necessary to: 1. have large quantities of starting DNA target 2. have purified target sequence DNA 3. know sequence of the 3’ end of each strand of the DNA target to be amplified 4. have dideoxynucleotide triphosphates to replicate the DNA 5. all of the above
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3 Name 2. ( 24 points total/ 6 pts each enzyme) 5 enzymes are listed below. Choose 4 of the 5 enzymes. For these, briefly state: i) What the enzyme normally does in vivo (3 pts) ii) Why it is useful in recombinant DNA technology (3 pts) (Note only four responses will be scored. Draw a line through the name of the enzyme you do not want scored, otherwise, your first 4 responses will be graded) Example: β -lactamase i) cleaves the lactam ring of antibiotics such as ampicillin, thereby
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This note was uploaded on 10/14/2008 for the course BIMM 100 taught by Professor Pasquinelli during the Spring '06 term at UCSD.

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practice midterm - BIMM 100 Midterm Exam Name Please write...

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