practice final

practice final - BIMM 100 Practice Final Name Please write...

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BIMM 100 Name Practice Final Please write your name on each page and look through the entire exam before beginning your answers. Your exam should have 12 pages. The exam has a total of 9 questions. Be certain to answer every question as directed. Please do not use pencil or white-out on your exam. Be certain that all of the work you want graded is written in permanent ink. Note that all sequences are presented in 5' -> 3' orientation and that a codon table and a restriction enzyme table have been provided as the last page of your exam. *This practice exam does not include the codon table from the MCB text (see p. 128 for codon table). Your score 1. 32 pts. 2. 18 pts. 3. 4 pts. 4. 6 pts. 5. 10 pts. 6. 18 pts. 7. 44 pts. 8. 14 pts 9. 34 pts. Total 180 pts.
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2 Name 1. (4 pts each/32 pts total). Circle the number corresponding to the correct response for the questions on this page and the next. A. Splicing of pol II transcribed RNAs joins 1. two intron sequences 2. two nascent transcripts 3. two DNA molecules 4. two exons 5. adjacent functional domains in proteins B. Which anticodon in tRNA recognizes the start codon in translation? 1. UAC 2. AUG 3. GUA 4. CAU 5. UGA C. Which of the following is a ribozyme-mediated process? 1. excision of group II introns 2. trans-splicing 3. RNA editing in intestinal tissue 4. excision of group III introns 5. splicing of pol III transcripts D. Which of the following is not used in DNAse I footprinting? 1. a radioactively labeled DNA fragment 2. a nitrocellulose filter 3. DNA binding protein(s) 4. DNAse I
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3 Name 1. (cont’d. 4 pts each/32 pts total). Circle the number corresponding to the correct response. E. Which of the following is a protein that is required for translation? 1. peptidyltransferase 2. RNA polymerase 3. ribosomal RNA 4. σ 54 5. aminoacyl-tRNA synthetase F. Which of the following substitutions could occur as a result of a single base change in the methionine codon? 1. Asp 2. Arg 3. Ala 4. Ser 5. Stop G. Which does not occur within the nucleus? 1. ribosome subunit assembly 2. debranching of excised lariat introns 3. 5' de-capping of mRNA transcripts 4. polyadenylation 5. non-homologous end joining H. Splice sites in pre-mRNA are marked by two universally conserved dinucleotide sequences 1. in the middle of the intron 2. at the branch site ‘A’ 3. at the ends of the introns 4. at the ends of the exons 5. at the exon:intron junction of the first exon
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4 Name 2. ( 6 pts each/ 18 pts total) Molecular biology is full of surprises. There are many cases in which a molecule is first characterized to function in one process or structure, and is subsequently found to have additional roles. For the three molecules listed below, state i) (3 pts.) its originally characterized function and ii) (3 points) a second, later characterized function for the molecule. e.g. Topoisomerase II –
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This note was uploaded on 10/14/2008 for the course BIMM 100 taught by Professor Pasquinelli during the Spring '06 term at UCSD.

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practice final - BIMM 100 Practice Final Name Please write...

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