07 summer midterm2

07 summer midterm2 - 1 Name (last, first) : 4 BlBC 100...

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Unformatted text preview: 1 Name (last, first) : 4 BlBC 100 University of California, San Diego. Dr. Lukas Buehler Summer 07 MIDTERM 2 (60 POINTS) YOUR SCORE: 1%; POINTS, YOUR GRADE _ All Students please read the following waiver: By signing this waiver. I give permission for this exam to be left for me to pick up in the lobby of York Hall 4070. lfl do not sign this waiver. I acknowledge that my exam will be available for pickup during office hours or class. _—_1 . Signature Date 1 PLEASE READ THE QUESTIONS CAREFULLY BEFORE ANSWERING! # 1. Draw a topology diagram of a stem and loop and a hair pin structure (label your drawings). In which class of nucleic acids are these motifs commonly found? Give one example of the functional use of a stem and loop structure that has been discussed in class and is related to l protein translation. p i 7‘ a ' CD Hui/i Mficntm {r7 Ski/M BCEWr I’m” 2. The major groove of DNA serves as protein binding site. The base sequence can be determined because Of unique distribution of matching binding sites. What types of non-covalent bonds are known to contribute to DNA-protein interaction? gig “’W‘l Moll“ Q WA gum“ (vim) @ hbw JWH Q From the list below, circle the protein(s) that interact with the major groove surface of DNA: '1) . (De La repre sor(transcript10n factor) Q E (_, striction enzyme) Histone 2A-ZB dimer (nucleosome organization) 2 Name (last, first) : 3. Draw a graph of the heat stability of DNA and show in the graph explicitly how to determine the melting temperature (Tm). Label both axes and indicate an approximate temperature r - _-_ for your denaturation curve. 4. Name the anomeric forms of D—glucopyranose and discuss the relevance that each form has on the tertiary structure of a linear, unbranched homo-polysaccharide. Name a matching polysaccharide for each anomer. ' V @/ A ‘7 WLC‘X «.7 use a» MW"? MW? @ (2 (2 5. Name three glycerophospholipids that would contribute to an overall negatively charged surface of a cell membrane. Assume pH = 7. (List only three) @ 0:327 791 h I fit ( Wt, "Pm CCMerL} P51.) / I? 3 Name (last, first) : 6. What is the relationship between the saturation level of fatty acids in phospholipids and ‘ melting or transition temperature of a lipid bilayer? 6p RAW M ’5‘ wiflh’“ WJWJDL “’5 [OW :k C; 1mm "7 low it,“ Based on your explanation, name one fatty acid that you would expect to find in high amounts in a membrane of an organism living in cold environments. I \ any/2C ate/Mt («Mme MM C72 7. List one difference and one similarity between an A- and B—DNA double helix (list only e lac: ?7+’L( Wfldukgi 9‘3mmcgw’ Q film: item/ti, bis/MA, limit/th @ Lciix rim/EL; WNN,WA]N gnu/{5 w m "b A 3.2 +71 + 8. Draw the orientation of a hospholipid in a monolayer at the water surface (air-water interface). Indicate the approximate dielectric value for each side of the interface. 4 Name (last, first) : —L___________ 9. Discuss the structural features of a cell surface receptor (plasma membrane protein) as an N- linked glycoprotein. Indicate the transmembrane orientation of this protein with respect to the glycosylation site. what amino acid sequence you would expect for this type of glycosylation, and the monosaccharide composition of the generic core oligosaccharide structure. 8p @ Mm and WWW (Q 10. Dimerization is a common feature of controling protein activity. What is the role of dimerization in proteins having a leucine zipper motif? What type of functions do these proteins have? Describe the structure of this motif, including known amino acid sequences patterns central to this motif. 6p ...
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This note was uploaded on 10/15/2008 for the course BIBC 100 taught by Professor Nehring during the Summer '07 term at UCSD.

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07 summer midterm2 - 1 Name (last, first) : 4 BlBC 100...

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