Exp 9 - strength of interaction between component and...

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TA: HSIEN-PO CHIU Oct 23 & 25, 2007 Exp 9 . Isolation and Isomerization of Lycopene from Tomato Paste Principle : 1. Composition of color: blue, yellow, and red 2. Color of organic compounds: occur w/ highly conjugated π bond system due to absorption of visible light for n π */ π π * electron transition. 3. The larger the conjugated system, the lower the energy, the lower the frequency (E=hv), the longer the wavelength ( λ =c/v) 4. Nutrients in Tomato: Lycopene (orange-red) for heart health, β -carotene (yellow-orange) for vision health Lycopene β -carotene OH trans -Vitamin A enzyme HO cis -Vitamin A O 11- cis -Retinal light O trans -Retinal Rhodopsin 5. Chromatography is the separation of a mixture by distributing its components between two phases: the stationary phase and the mobile phase. The stationary phase remains fixed in place, while the mobile phase flows through it, carrying components of the mixture along with it (called eluent). The stationary phase (called adsorbent) acts as a ‘brake’. The strength of the brake depends on the
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Unformatted text preview: strength of interaction between component and stationary phase. 6. Normal phase chromatography: polar adsorbent w/ nonpolar eluent Reversed-phase chromatography: nonpolar adsorbent w/ polar eluent Procedure : follow procedure on p.78-79 Results : 1. Weight of tomato paste 2. Weight of lycopene 3. percentage of lycopene 4. Weight of β-carotene 5. Percentage of β-carotene Q&A 1. Why did you never allow the column to go dry after you started the procedure? (A) it can create irregularities in the column (B) side reactions can occur between the stationary phase and air (C) it will slow the process of separation (D) it can cause rapid decay of the component mixture 2. Which will best help you separate the compounds with similar polarities? (A) increase the polarity of your solvents (B) take smaller fractions (C) decrease the amount of alumina (D) decrease the polarity of your solvents...
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This note was uploaded on 10/16/2008 for the course CHEM 201 taught by Professor Richard during the Fall '07 term at SUNY Buffalo.

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