bioinformatics lab

bioinformatics lab - colocalize with the Crumbs protein...

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Lindsay Wasicek BIO 315 L55 10/14/08 Bioinformatics Questions 6. They don’t match entirely; this makes sense because they are in different organisms, therefore their structure/domains and functions will be a little different. 11. There are 81 PDZ domain-containing proteins in the Drosophila melanogaster genome. There are 151 domains; this number is larger than the 81 proteins because proteins can have more than one domain. 15. Yes, there are common secondary features and common tertiary structures among all three proteins. It looks like there are one-two alpha helixes and about five beta sheets in each domain cartoon. This makes sense because if the structures of these domains are similar in each protein, they are likely to perform similar functions. 24. A) I would predict that the hINADI would be located in the apical region of epithelial cells. I would also predict that hINADI would perform a similar function to DLT, it would interacts and
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Unformatted text preview: colocalize with the Crumbs protein working in a complex together to generate and maintain epithelial cell polarity in humans. B) Experiments could be done to test the location of and role of hINADI in humans such as immunofluorescence. Other tests could be conducted to test the polarity of the epithelial cells in the presence and absence of the protein. We could also test for specific activity, like we did in the ALP lab. C) The first PDZ domain of DLT is necessary for DLT’s function, binding to Crumbs. Since hINADI’s function is also hypothesized to bind to Crumbs, I would hypothesize that hINADI’s PDZ domain will be the same or very similar to that of DLT’s. This was shown in the WorkBench program where the amino acid sequence of the two had a large corresponding region beginning with amino acid 250 in the sequence....
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This note was uploaded on 10/16/2008 for the course BIO 315 taught by Professor Howard during the Fall '08 term at Wisc La Crosse.

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