Sample Final

Sample Final - 1 of 3 Name Last Score(200 First Student ID...

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1 of 3 Name: Last, First Student ID Score (200) Sample Final Exam (Bis102-002) pH = pK a + log {[base]/[acid]} Δ G = Δ H - T Δ S v o = {V max [S]} / {K m + [S]} pK a of the side chain R-groups are: D (3.9), E (4.2), H (6.0)' C (8.3), Y (10.1), K (10.5), and R (12.5). pK a of amino acid α -amino group (9.60) and α -carboxyl group (2.10). pK a of N-terminal oligopeptide a-mino group (7.40) and C-terminal a-carboxyl group (3.6). 1. An enzyme catalyzed reaction both in the presence and absence of reversible inhibitor "I". As "I" turned out to be a competitive inhibitor, (a) what could be observed in a Lineweaver-Burk plot was made in terms of Vmax? (b) If the kinetic experiments had been re-done using half as much enzyme in every reaction (test tube), what would be the Vmax and Km compared to the original Vmax and Km values? (15pts). 2. Calculate the net charge, to the nearest 0.01, of the peptide D-E-K-C-R-P at pH 8.10. You may use the back of this paper for calculations. There is no partial credit. (15 pts.) 3. How many moles of HCl are required to lower the pH of 600 ml of 0.300 M glycine buffer, pH 9.80 to a final pH of 2.40? You may use the back of this paper for calculations. There is no partial credit. (15 pts.)
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This note was uploaded on 10/19/2008 for the course BIS 102 taught by Professor Hilt during the Summer '08 term at UC Davis.

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Sample Final - 1 of 3 Name Last Score(200 First Student ID...

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