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Unformatted text preview: increases. The polarity and solubility of these pigments, ranked from least to greatest go in the order of Beta-carotene, Chlorophyll a, and Chlorophyll b. 7. The phycocyanins were more negatively charged than the phycoerythrins. The DEAE was positive and the Tris solutions were more and more ionic as the concentration increased, and as it dripped through the column, the stronger solutions would pull down the proteins with them. Since the phycoerythrins came down earlier, they were pulled down by a weaker charge and were thus less negative than the phycocyanins. 8. Pigment Wavelength in blue region (400-500 nm) Wavelength in red region (600-700 nm) Chlorophyll a 435 665 Chlorophyll b 455 615...
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- Fall '07