Micro305_Lab Report 3

Micro305_Lab Report 3 - Micro 305 April 4 2007 Lab Report 3...

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Micro 305 April 4, 2007 Lab Report 3: Pure Culture Technique Introduction Bacteria are found all over this planet, but are rarely isolated from each other in nature. Scientists have developed a number of techniques to try and isolate different types of bacteria from a sample. They do this in order to perform tests on only one type of bacteria. One of the most common methods for isolating pure cultures is the streak plate method. In this method, the sample is strategically streaked onto an agar plate to try and isolate all types of bacteria in the sample. In a lab to determine unknown bacteria, students used this method to isolate the two different types of bacteria given. One must be sure to work around a flame, and use aseptic technique, as to not contaminate the sample with outside bacteria. Materials and Methods The materials can be located on page 73 of Benson’s Microbiological Applications (10th ed., short version by Brown). The method used is the quadrant streak method B, found on page 77 in Benson’s Microbiological Applications (10th ed., short version by Brown).
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