2081433883

2081433883 - Topic 4. Thermodynamics & Enzyme Catalysis...

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Topic 4. Thermodynamics & Enzyme Catalysis Ch. 3 Thermodynamics : study of the changes in energy accompanying events in the physical universe. Review the first and second law of thermodynamics. Of what significance is thermodynamics it to biology, and what can be predicted? Free energy and its relationship to the spontaneity of chemical processes. All spontaneous energy transformation must have a negative G . Are living cells in an equilibrium state, or a steady state? How do cells benefit from coupled biological reactions? Review characteristics of enzyme: Enzymes are organic catalyst. Why won’t inorganic catalysts serve the same purpose for a cell? Activation energy vs. transition state in enzymatic reaction. Mechanisms of metabolic regulation: feedback inhibition, activators, etc. . Activation energy Free energy G Change in free energy G Endergonic reaction Exergonic reaction Enthalpy Entropy Conformational change
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1 BSCI230-Lecture4 Enzymes,Catalysis,Kinetics Topic 4: Chp. 3 G Chitin subunit: Tertiary structure - native -S-S- R -S - Experimental steps showing protein unfolding and refolding A. B. C. Protein Denaturating Causes Interfere mostly with tertiary structure • Heat (unfold, hydrophobic region exposure) • Ionic detergents (SDS, ionic bonding effects) • pH (how or low, may ppt. proteins) • Oxidizing/reducing agents (-SH agents can block oxidation state formation) Also enzymes may not be able to undergo CONFORMATIONAL CHANGE Thermodynamics – study of changes of energy in energy accompanying event in the physical universe . First Law of Thermodynamics: energy cannot be created or destroyed, it can be converted from one form to another. Second Law of Thermodynamics : things tend to proceed "downhill" from ordered to less ordered, and in the process lose some ability to do work (energy gone to other forms not now recoverable)
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2 ENERGY ( G) Molecules of cell posses energy because of • Their vibrations, rotations, etc. • Energy (
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This note was uploaded on 10/22/2008 for the course BSCI 207 taught by Professor Higgins during the Spring '08 term at Maryland.

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2081433883 - Topic 4. Thermodynamics & Enzyme Catalysis...

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