Exp 1 conclusions - -The data from this experiment shows...

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-The data from this experiment shows that the B-G spectrophotometer was found to be capable of being brought to zero by a slit width of 2mm or less at a wavelength of 210nm (at 210nm, 0.68mm was the slit width). Therefore, the operational slit width of the spectrophotometer was acceptable. This is important because as the slit width increases, the spectral purity of light decreases (and the intensity of the light increases). Similarly, a larger slit width would result in a higher spectral bandwidth value. -SBW is used to express the spectral purity of light; as the SBW value increases, the spectral purity of light decreases. The accuracy of absorbance measurement on a spectrophotometer decreases as the purity of light decreases. Therefore, a higher SBW value will result in less accurate absorbance measurements. -The accuracy of absorbance measurements on a spectrophotometer can be determined by considering the ratio of SBW/NBW. When the spectral bandwidth is less than one-tenth the value of the natural bandwidth of the absorbance band, deviation from the true
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This note was uploaded on 03/19/2008 for the course BMB 471 taught by Professor Bowlby during the Spring '08 term at Michigan State University.

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