Unit A Report I Answers

Unit A Report I Answers - Sec. 001 Report I, Unit A...

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Sec. 001 Report I, Unit A Spectrophotometer # 10 BMB 471 – Report I – Experimental Unit A Answers 1. Lab Exercise A.5.a.(1) a. Give values, including calculated values, (wavelength, slit width, dispersion, SBW, TBW) i. Table attached separately as Table 1-1. b. Graph SBW versus wavelength. i. Graph attached separately as Figure 1-1. c. Is the operational slit width of your spectrophotometer acceptable? i. Yes. d. What is the basis for your decision? Comment on the spectral purity of the light in your spectrophotometer at various wavelengths. i. The data from this experiment show that the B-G spectrophotometer was found to be capable of being brought to zero by a slit width of 2mm or less at a wavelength range of 210- 800nm. Therefore, the operational slit width of the spectrophotometer was acceptable. This is important because as the slit width increases, the spectral purity of light decreases (and the intensity of the light increases). Similarly, a larger slit width would result in a higher spectral bandwidth value. Low spectral purity of light negatively affects the ability of the spectrophotometer to accurately take measurements of absorbance. 2. Lab Exercise A.5.a.(2)(i) a. Plot your data as an absorption spectrum. i.
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This note was uploaded on 03/19/2008 for the course BMB 471 taught by Professor Bowlby during the Spring '08 term at Michigan State University.

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Unit A Report I Answers - Sec. 001 Report I, Unit A...

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