Mid 1 Winter 2008

Mid 1 Winter 2008 - 1 of 2 BIS 102 Winter, 2008 K. Hilt...

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1 of 2 BIS 102 Name__________________________________________________ Winter, 2008 Last First K. Hilt First Midterm Score (100): ___________ Equations: pH = pK a + log {[b]/[a]} K a = x 2 /(y-x) K b = x 2 / (y-x) pH = (pK a 1 + pK a 2 )/2 (K a )(K b ) = 1 x 10 -14 F = (q 1 q 2 ) / ε r 2 G = H - T S Amino acid pK a ’s: α -carboxyl group (2.1), α -amino group (9.6) side chains: D (3.9) E (4.2) H (6.0) C (8.3) Y (10.1) K (10.5) R (12.5) Oligopeptide pK a ’s: C-terminal carboxyl group (3.6), N-terminal amino group (7.4) 1. The amino acid glycine is used as a “trailing ion” in gel electrophoresis. It starts out in a pH 8.3 reservoir buffer, then moves into a pH 6.8 gel region, and then moves into a pH 8.8 gel region, before entering a pH 8.3 reservoir at the bottom of the apparatus. This is diagramed below, on the right. Please answer the following questions, regarding this gel system.
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Mid 1 Winter 2008 - 1 of 2 BIS 102 Winter, 2008 K. Hilt...

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