BMB 495 Veille Summary Report

BMB 495 Veille Summary Report - BMB 495 2/26/08 Summary...

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BMB 495 2/26/08 Summary Report for 2/26 – Vieille Title and Journal Reference: Stols, L., & Donnelly, M. I. (1997). Production of Succinic Acid through Overexpression of NAD + -Dependent Malic Enzyme in an Escherichia Coli Mutant. Applied and Environmental Microbiology , 63 (7), 2695-2701. Hypothesis: To evaluate the possibility of constructing an alternative pathway in E. Coli for producing C 4 -dicarboxylic acids (Succinic, fumaric, and malic acids) from glucose, utilizing overexpressed malic enzyme catalysis to carry out reductive carboxylation of pyruvate to malate. Background/Significance: Researchers often utilize metabolic engineering to create new or alternate metabolic pathways in an organism which results in production of a novel or commercially useful chemical. This is often done by amplifying an existing organismal pathway or introducing an alternate pathway that uses one or more non-natural or modified enzyme(s) (through genetic modification). In this case, Stols and Donnelly propose an alternative pathway in the production of C 4 -dicarboxylic acids from glucose. These dicarboxylic acids have significance in that they can be converted into 1,4- butanediol, tetrahydrofuran, and g-butyrolactone, which are utilized by the petrochemical industry to produce solvents, polymers, and additives. In normal E. Coli , glucose is converted into succinic acid through a pathway involving the transformation of PEP (formed from pyruvate). The researchers propose that succinic acid can alternatively be generated as an end product of this pathway by converting pyruvate directly to malate through reductive carboxylation catalyzed by overexpressed
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malic enzyme. In this case, the normal metabolism of pyruvate (involving PEP) has been blocked in the organism. Methods:
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BMB 495 Veille Summary Report - BMB 495 2/26/08 Summary...

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