Assessment :Format (1%)Methods (2%)Calculation/ Drawing/Data/ Results (2%)Discussion (3%)Conclusion (1%)Ref. (1%)Total (10%)EXPERIMENTTITLEINTRODUCTION TO MICROSCOPYGROUP MEMBER MATRIX NO.1.MUHAMMAD FADZLIH BIN BAHARUDINFS171100362. NUR ANIS SYAHIRAH BINTI ASHARIFS171100793. NOOR SYAFIKAH BINTI ABDUL RAHIMFS171101824. DATE OF EXPERIMENT :10 JULY 2017DATE OF SUBMISSION :17 JULY 2017DEMONSTRATOR :MS. JUVITA WONG JUNIntroductionSB0011BIOLOGYPRACTICAL 1LABORATORYREPORT
The first microscope to be developed was the optical microscope, although theoriginal inventor is not easy to identify. An early microscope was madein 1590 in ,Middelburg . Two eyeglass makers are variously given credit: HansLippershey and HansJanssen. Giovanni Faber coined the name microscopefor Galileo Galilei's compoundmicroscope in 1625. Microscope is a tool usedto enlarge images of small objects that are hard to study with bare eyes. Thecompound light microscope, which is going to be used in this lab activity, is aninstrument with two lenses and various knobs to focus the image. In this lab, wewill learn about the proper use and handling of the microscope.ObjectivesAt the end of this practical, we should be able to:1.Identify the name and the function of the basic parts of the compoundlight microscope.2.Learn how to focus an object with the compound microscope.3.Measure the diameter of the field of view of each magnification power.MaterialsCompound light microscopeSlidesMarker penLetter ‘e’ from newspaperProcedureA) Identification of parts
Microscope should obtain from the cabinet and place it securely on the table.Identify the following parts on the microscope.1.Eyepiece(ocular lens): Contains a magnifying lens2.Body tube: Keeps proper distance between the eyepiece and the objectives.3.Arm: Supports the body tube4.Nosepiece: Holds the objectives and can be rotated5.Scanning power objective: Hold 4x lens used to view the whole slide6.Low-power objective: A shortest objective that magnifies 10X (4X sometimes)7.High-power objective: A longest objective that magnifies 40X (100X sometimes)8.Oil immersion objective: Holds a 95x(to 100x) lens and is used in conjunction with immersion oil to view objects with the greatest magnification9.Coarse-adjustment knob: Moves the body tube to focus the image10.Fine-adjustment knob: Moves the body tube slightly to sharpen the image11.Diaphragm or diaphragm control lever: Contol amount of illumination used to view the object12.Light source: An attached lamp that directs a beam of light up through to view the object 13.Base: Supports the microscope14.Stage: Supports the slide being observed15.Stage clips: Holds the slide16.Mechanical stages: A moveable stage used in accurate positioning of the slide 17.Mechanical stage control knob: To control foward/reverse movement, and the other controls right/left movementB)Focusing the Microscope
Lowest Power1.The nosepiece is turn so that the lowest power lens in straight alignment over the stage.