BIS 101 Midterm II Answer Key

BIS 101 Midterm II Answer Key - Name: _ BIS101-01, Fall...

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Name: ______________________________ ID:_______________________________ BIS101-01, Fall 2007 Dr. D. J. Kliebenstein, Instructor Midterm Exam II Please print your name and ID number on each page of the exam. The exam has 12 questions and a total of 120 points. For full credit, show all of your work. AUTHORIZATION FOR PUBLIC DISTRIBUTION OF GRADED PAPERS OR EXAMINATIONS I, _______________________________, student ID No.__________________________________: authorize the University to publicly distribute my graded exam (e.g., handed out in class or left in an exam bin) for BIS101-01. If I do not sign, then I must see the instructor to pick up my exam. Signed: ________________________________ Dated: _______________ 1
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Name: ______________________________ ID:_______________________________ 1 __________ 2 __________ 3 __________ 4 __________ 5 __________ 6 __________ 7 __________ 8 __________ 9 __________ 10 __________ Total _________ 2
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Name: ______________________________ ID:_______________________________ 1. (15 points) Complete the following Table, assuming that reading is from left (5’) to right (3’) and that the columns represent transcriptional and translational alignments. For DNA, use ACTG or N where N represents any possible nucleotide. T G G T C G T T N T A A DNA double helix A C C A G C A A N A T T U G G U C G U U N U A A mRNA A C C A G C A A N no anticodon tRNA anticodon amino acids TRP SER PHE or LEU STOP incorporated into protein Which of the above rows is the non-coding strand? The first DNA strand is the coding strand. Which of the above rows is/are not completely chemically linked from left to right? The t-RNA anticodon row is not chemically linked. 3
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Name: ______________________________ ID:_______________________________ 2. (16 points) In lecture, we went over a number of sequences and pieces of information required to generate a functional prokaryotic gene. Use the line below as a DNA element and diagram a prokaryotic gene including all information necessary to make a functional protein. State if each element functions as DNA, RNA or Protein? 3) Name and describe the two mechanisms that prokaryotes use to terminate transcription. (6 points) Direct termination – this involves a stretch of RNA that is homologous in a head to head orientation such that it can form a stem-loop structure. When this structure forms, the RNAP falls off the RNA and you get termination. Rho dependent termination – this involves the binding of the Rho protein to the RNA at a specific
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BIS 101 Midterm II Answer Key - Name: _ BIS101-01, Fall...

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