BMB461_Exam_4_key - BMB 461 Midterm IV-2006 Name Student...

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Unformatted text preview: BMB 461 Midterm IV-2006 Name Student Number Signature This exam consists of 10 pages with 50 questions worth 2 points each ---- Be sure you have all 10 pages. Total Points = 100. The answers to the questions must be placed on the bubble sheet. 1. The proton motive force generated by the electron transport chain: A. includes a pH gradient component. B. Includes an electrical-potential-gradient (charge separation) component. C. Is used for active transport processes. D. Is used to synthesize ATP. E. Has all of the above. XXXX 2. The overall yield of ATP from glucose is lower when cells using the glycerol- phosphate shuttle than cells using the malate-aspartate shuttle. Why? A. Cells using the glycerol-phosphate shuttle only degrade glucose anaerobically B. The use of glycerol-3-phosphate shuttle reduces the overall yield by 2 (two) moles of ATP because of its entry point in the electron transport chain. C. Cells using the malate-aspartate shuttle to transfer reducing power of NADH into the mitohondrial transport the full reducing power of NADH from cytosol to the mitochondria. D. A and B E. B and C XXXX 3. The electron transport chain contains four membrane complexes, but only three of them participate directly in proton pumping, thus establishing an electrochemical gradient across the inner mitochondrial membrane. Select the answer that lists the three proton pumping complexes. A. Complexes I, II, and III B. Complexes I, II and IV C. Complexes II, III and IV D. Complexes I, III and IV. XXXX 4. Which of the following sequences is correctly ordered from least to most affinity for electrons? A. Cytochrome c >Coenzyme Q > Cytochrome a B. FAD > NAD >Coenzyme Q C. Cytochrome a >Cytochrome c > FAD > Coenzyme Q D. NAD > Cytochrome a >Coenzyme Q E. Cytochrome a> Cytochrome c > NAD F. NAD > CoQ > Cytochrome a XXXX 5. Match the following chemical to its effect on electron transport and oxidative phosphorylation: Dinitrophenol A. Stops NAD +-linked oxidation although FAD-linked oxidation can still occur if a suitable substrate is added,. In these conditions, P/O ratio would not be higher than 2. B. Electron transport can resume if a suitable substrate is added but P/O ratio cannot be higher than 1. C. ATP/ADP translocase is inhibited. D. Oxygen uptake increases, protons can diffuse freely across the inner mitochondrial membrane disrupting the proton gradient. XXXX E. ATP synthesis stops, immediately followed by a stop in oxygen uptake. 6. Which of the following is the PRIMARY effect of a deficiency in superoxide dismutase? A. Damage to cellular components due to an increase in hydrogen peroxide B. Damage to cellular components due to an increase in the concentration of oxygen radicals XXXXX C. Disruption of the proton gradient across the inner mitochondria membrane D. Cytochrome c oxidase is inactivated E. None of the above 7. Respiratory coupling or control is defined as: A. Regulation of oxygen consumption by oxaloacetate availability B. Regulation of electron transport by availability of NAD B....
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BMB461_Exam_4_key - BMB 461 Midterm IV-2006 Name Student...

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