08 Lecture

08 Lecture - 10/21/08 Microarrays Look @ gene expression on...

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Unformatted text preview: 10/21/08 Microarrays Look @ gene expression on a large scale (entire genome at once) under different conditions Ex: what genes are expressed more if grown in glucose vs in galactose Gene Inactivation Knockout (KO) a gene to see function that gene had o Use a KO cassette Consists of a drug/nutritional marker (Ex: KanMX gives resistance to G418) + specific flanking regions Flanking regions are added using oligos w/ specific sequences o Flanking regions will guide the KO cassette to specific place in chromosome o Flanking regions will cross over, swapping out the gene of interest with your drug/nutritional marker gene o You know which cells have KOd genes because they have that marker (such as resistance to G418) o This method only works for non essential genes (75% in yeast) Regulated Promoter used for essential genes o Control transcription by swapping out the wild type promoter with a promoter you can control o Promoter is introduced same way as KOing a gene o...
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This note was uploaded on 10/25/2008 for the course BIMM 100 taught by Professor Pasquinelli during the Fall '06 term at UCSD.

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08 Lecture - 10/21/08 Microarrays Look @ gene expression on...

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