Chapter-25-Notes

Chapter-25-Notes - 1. Ch. 25 High-Performance(-Pressure)...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
1. Ch. 25 High-Performance(-Pressure) Liquid Chromatography (HPLC)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2. Small particles give high efficiency but require high pressure Azo compounds run on (a) 10- µ m-diameter and (b) 5- µ m-diameter silica particles. Plate height as a function of flow rate for stationary-phase particle size of 10, 5, and 3 µ m. N ~ 3,500 L (cm) N = number of plates d p (mm) L = column length d p = particle diameter
Background image of page 2
3.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4. The Column Guard column rem st, particles, irreversibly adsorbe ties. Porous frits distribute liquid evenly across the area of the column. oves du d impuri
Background image of page 4
5. The Stationary Phase Most common is spherical, microporous silica particles. Scanning electron micrographs of silica particles. (a) aggregate of spherical particles with 50% porosity and surface area of 150 m 2 /g. (b) Spongelike structure with 70% porosity and 300 m 2 /g. Nominal pore sizes are ~10 nm, but distribution is greater in b, which also dissolves more readily in base. Schematic of silica gel
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 19

Chapter-25-Notes - 1. Ch. 25 High-Performance(-Pressure)...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online