final_2008_key

final_2008_key - 1 BIS 101 - 002 FINAL June 10, 2008 READ...

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Unformatted text preview: 1 BIS 101 - 002 FINAL June 10, 2008 READ THE FOLLOWING INSTRUCTIONS: Write your name legibly on each page of this exam. All work must be done in ink . Show all your work. Points will be subtracted for incorrect answers in multiple-choice questions. However, you will not get a negative total score for such a question. This exam is worth 200 points. You have 110 min. to complete the exam. Authorization for public distribution of graded examination I, ____________________________________, ID _________________________ authorize the University to publicly distribute my grated examination for BIS 101-003. Date_______________________________ Signed: _____________________________ Good Luck! 2 Name:______________________________ P a g e : 3 ________ 4 ________ 5 ________ 6 ________ 7 ________ 8 ________ 9 ________ 10 ________ 11 ________ 12 ________ 13 ________ 14 ________ 15 ________ 16 ________ Total ________ Bonus_________ 3 Name:______________________________ (1) You have a Drosophila stock with phenotype A. Design an experiment to decide if it is an AA or Aa genotype. (6 points) The stock should be test testcrossed with an aa line. If it is Aa, progeny will segregate 1A : 1a phenotypes If it is AA, all progeny will be the A phenotype (2) A pure-breeding strain of squash that produced a disk-shaped fruits was crossed with a pure-breeding strain having long fruits. The F 1 had disk fruit, but the F 2 segregated for three phenotypes, disk fruit, long fruit, and sphere fruit. The following numbers of phenotypes were observed: Disk 270 9 Sphere 178 6 Long 32 1 Use the following chart for a dihybrid F 2 to explain these results. Assign phenotypes to the four genotypes and indicate their theoretical frequencies. Assume complete dominance throughout. (6 points) A-B- disk 9 A-bb sphere 3 aaB- sphere 3 aabb long 1 ( 3 ) Five histones are known. (a) Four of them form the core of the nucleosome. Name the four. (4 points) H2A, H2B, H3, and H4 (b) Name the remaining histone. What is its role? (2 points) The remaining histone is H1. It assists the folding of 30 nm solenoid. 4 Name:______________________________ (1) Drosophila females heterozygous for sn/sn + cv/cv + and v/v + were testcrossed. The following phenotypes were obtained in progeny. (a) sn cv v 3 (b) sn + cv v 392 (c) sn + cv + v 34 (d) sn + cv v + 61 (e) sn cv v + 32 (f) sn cv + v 65 (g) sn cv + v + 410 (h) sn + cv + v + 3 Total 1000 What is the correct order of these three genes and what are the genetic map distances between sn and cv, sn and v , and cv and v ? (8 points) Compute interference of these intervals? (4 points) The parental chromosomes are: sn + cv v and sn cv + v + (a) sn-cv = 3+34+32+3/1000 = 7% (b) sn-v = 3+61+65+3/1000 = 13.2% (c) cv-v = 34+61+65+32/1000 = 19.2% The map is 7% 13.2% ___ cv ______________ sn ____________________ v ___ Classes (a) and (h) are doubly recombinant I = 1- 0.006/0.07 x 0.132 = 0.35 5 Name:______________________________...
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final_2008_key - 1 BIS 101 - 002 FINAL June 10, 2008 READ...

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