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final_key - 1 BIS 101 - 003 MIDTERM II May 15, 2005 READ...

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1 BIS 101 - 003 MIDTERM II May 15, 2005 READ THE FOLLOWING INSTRUCTIONS: Write your name legibly on each page of this exam. All work must be done in ink . Show all your work. Points will be subtracted for incorrect answers in multiple-choice questions. However, you will not get a negative total score for such a question. This exam is worth 200 points. You have 110 min. to complete the exam. Authorization for public distribution of graded examination I, ____________________________________, ID _________________________ authorize the University to publicly distribute my grated examination for BIS 101-003. Date_______________________________ Signed: _____________________________ Good Luck!
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2 Name:______________________________ P a g e : 3 ________ 4 ________ 5 ________ 6 ________ 7 ________ 8 ________ 9 ________ 10 ________ 11 ________ 12 ________ 13 ________ Total ________ Bonus_________
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3 Name:______________________________ (1) You have a Drosophila stock with phenotype A. Design an experiment to decide if it is an AA or Aa genotype. (8 points) The stock should be test crossed with aa line. If it is Aa, progeny will segregate 1A : 1a phenotypes If it is AA, progeny will all be the A phenotype (2) An F 1 trihybrid was testcrossed to v v ct ct cv cv. The phenotypic classes and % recombination frequencies between the v (vermilion eyes), cv (crossveinless) and ct (cut wing edge) loci are given below: (a) v ct + cv + 580 (b) v + ct cv 592 (c) v ct + cv 45 (d) v + ct cv + 40 (e) v ct cv 89 (f) v + ct + cv + 94 (g) v ct cv + 3 (h) v + ct + cv 5 Total 1448 The linkage among the three loci is: 13.2% 6.4% v ─────────── ct ─────── cv (a) Which of these eight classes are doubly recombinant ? (4 points) (g) and (h) (b) Calculate interference in the v - cv interval. (8 points) Observed freq. doubles 8/1448 0.0055 I = 1 - ───────────────── = ───────────── = ───── = 0.34 Expected freq. doubles 0.132 x .064 0.0084 8 or 1 - ────── = 0.34 12.1
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4 Name:______________________________ (1) A pure-breeding strain of squash that produced a disk -shaped fruits was crossed with a pure-breeding strain having long fruits. The F 1 had disc fruit, but the F 2 segregated for three phenotypes, disk fruit, long fruit, and sphere fruit. The following numbers of phenotypes were observed: Disk 270 9 Sphere 178 6 Long 32 1 Use the following chart for a dihybrid F 2 segregation to explain these results. Assign phenotypes to the four genotypes and indicate their theoretical frequencies. Assume complete dominance throughout. (8 points) A-B- disk 9 A-bb sphere 3 aaB- sphere 3 aabb long 1 (2) In mouse, only the maternal gene H19 is always expressed. This is an example of: Circle the most correct answer. (4 points) (a) position effect variegation, (b) somatic mutation, (c) maternal imprinting, (d) paternal imprinting, (e) none of the above. (3)
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This note was uploaded on 10/26/2008 for the course BIS 101 taught by Professor Simonchan during the Spring '08 term at UC Davis.

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final_key - 1 BIS 101 - 003 MIDTERM II May 15, 2005 READ...

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