3 - Protein Purification.pdf

3 - Protein Purification.pdf - Part 2 Purification...

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1/30/2017 1 Proteins Part 2 Purification Techniques Protein/peptide Purification To be able to study a protein or enzyme in detail you need to purify it! Protein Source Separation Techniques Size Charge Hydrophobicity Specific interaction Protein Detection Detection of all protein Detection of target enzyme/enzyme assays 3 Example: Methyl - coenzyme M Reductase (MCR ) This enzyme will be used to give practical examples of the theory and techniques introduced Methanogenic Archaea: Production of CH 4 4 CH 4 CO 2 H 2
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