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1/30/20171ProteinsPart 2PurificationTechniquesProtein/peptide PurificationTo be able to study a protein or enzyme in detail you need to purify it!•Protein Source•Separation Techniques•Size•Charge•Hydrophobicity•Specific interaction•Protein Detection•Detection of all protein•Detection of target enzyme/enzyme assays3Example: Methyl-coenzyme M Reductase (MCR)•This enzyme will be used to give practical examples of the theory and techniques introduced•Methanogenic Archaea: Production of CH44CH4CO2H2
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