3/18/2017 3 𝑉0=𝑉?𝑎𝑥[?]𝛼𝐾?+ 𝛼[?]𝑉0=𝑉?𝑎𝑥[?]𝛼𝐾?+ [?]Inhibition Studies: Pure Noncompetetive (α=α’) 9 Irreversible Inhibition •Inhibitors that bind covalently with or destroy a functional group on an enzyme that is essential for the enzyme’s activity. •Suicide Inactivator/Mechanism-Based Inactivator 10 III) Second-Order Reactions ? + ? ⇄ ? + ?•Sequential/single-displacement reactions •Both A and B are bound to the enzyme before the reaction can occur. •“Ordered” or “random”•Double-displacement (ping-pong) reactions •Proceeds via formation of a covalently modified enzyme intermediate 11 Single-displacement/ sequential Second-Order Reactions 12
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