4 - Protein Characterization.pdf

4 - Protein Characterization.pdf - Part 3 Characterization...

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1/31/2017 1 Proteins Part 3 Characterization Protein Characterization The protein has been purified, what’s next? Questions: How is the methane produced? What amino acids are important for this activity? What does the active site looks like? Is there a cofactor present/other modifications? Reaction mechanism Options: Study reaction kinetics (enzymology notes) Get amino acid sequence Determine exact mass of protein Compare theoretical mass (from sequence) with actual mass Determine 3D structure (Part 1 Notes) 3 3.1) Sequencing Procedure 1) Obtain amino acid composition 2) Determine which amino acid is present at the N-terminus 3) Break disulfide bonds if present 4) Sequencing 4
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