Bacterial Unknown Guide (1).doc - Inshort::a (keepitsafe....

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 Bacterial Unknown Guidelines – DUE July 27 th In short:  You will be provided with a TSB containing a mixture of two species of bacteria: a  Gram positive and a Gram negative (keep it safe). Isolate colonies of each onto agar plates of  complex media (TSA). Examine your bacteria’s colony characteristics and colony morphology  using your fancy simple staining and observational skills. Use the lab manual and your  experience to develop a flow chart that will aid you as you narrow down the ID of your  unknowns (submit it for review or… don’t.) Maintain viable strains throughout the process,  preferably on TSA slants. Use additional stains, differential media, and available chemical tests  to positively determine your bacterial species. Write a short bio for each of your two organisms  and a mini-report for each (about 1 page for each relating interesting recent  research/developments citing two sources per organism.) Timeline : Unknowns will be distributed on  June 20 th . You will have at least five weeks for the  entire process including several dedicated lab sessions and “open labs” (specific days and times  TBD).  All experiments must be completed by Wednesday, July 26 th !!!  Your reports are due  by the end of class on  Thursday, July 27 th . Reports can be turned in early to the instructor  during the designated office hours or by appointment.    Isolation of organisms: Each person will receive two bacterial organisms mixed together in a tryptic soy broth.  One  organism will be Gram positive the other will be Gram negative.  Each student will be given the  2 TSA plates for isolating the two from each other after the initial isolation attempt.  Altering  growing (physical) conditions is also allowed. Each student is responsible for his/her own  unknowns, and all work is to be performed  independently .   Identification of organisms: After separation, the organisms should be maintained on plates or slants AND in a liquid  medium for further testing.  BEFORE blindly testing the organisms with every media in the lab,  everyone should devise a key for their possible organisms (from a simple stain one can  distinguish morphology).   Only those tests that would benefit the identification should be  attempted . Points will be deducted for superfluous tests. After a tentative identification has been established, the person should confirm their ID of the organism to me.  I will suggest further tests
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