BME 201 HW #3.docx - September 19th 2016 Professor...

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September 19 th , 2016 Professor Kaiming Ye Homework #3 1. Bacterial and Eukaryotic transcription have several differences, especially in the enzymes that carry out transcription. Eukaryotes use RNA polymerase I, II, and III during transcription, but bacteria need only one of these. This one RNA polymerase also needs only one extra protein, a sigma factor, for in vitro transcription. Eukaryotic RNA polymerases, however, need many proteins, known collectively as general transcription factors. Furthermore, these factors differ in the variety of processes they carry out. 2. a. mRNA: codes for proteins b. rRNA: components of ribosomes c. snRNA: splicing of RNA transcripts d. SnoRNAs: chemical modification of other RNA’s 3. False, because the sigma factor is only needed during the initial synthesis of RNA. The sigma factor will aid in the synthesis of only a few nucleotides, and then detach from the RNA polymerase. This causes a structural change in the enzyme, allowing it to synthesize more nucleotides without the presence of a sigma factor.
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