lab test 2.docx - Experiment 3 Cell Membrane Transport 1...

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Experiment 3: Cell Membrane Transport 1
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Abstract: In this experiment I learned how molecules traverse the cell membrane. I also demonstrated passive differential transport by using dialysis tubing and studying the movement of iodine, glucose, and starch. I then tested their results with Benedict’s reagent. I analyzed the effect of temperature on the rate of potassium permanganate diffusion. For a final test I used osmosis to determine the tonicity of potatoes in different saline solutions. Introduction : The purpose of this experiment is to demonstrate the transport of molecules across cell membranes, understand diffusion, and how temperature affects diffusion. Also to understand osmosis and how it relates to tonicity of solutions. Finally, I will learn how to test for the presence of starch and sugars in different solutions. Hypothesis: Dialysis test- I predict that the Solution in cup 1 will not change, but that the solution for in the dialysis tubing will change to blue. Benedicts Reagent test: I think that the IKI Solution will not change since it did not in the first test. I think that the solution in the dialysis bag will change to a color between orange and brown. And the distilled water will change from a color between orange and brown. Diffusion of KMnO4: I think that the hot and cold water will change the diffusion but I do not think that the room temperature one will. Potato Dimensions: I believe that the potato that is in the distilled water will soak up all the water and become bigger (hypotonic) and that the potato that is in the sodium mixture will become smaller (hypertonic). Procedure: Materials: Distilled water Drinking glasses, clear Cups, Styrofoam Stovetop or hot plate, and pan or deep skillet for water bath Ice Fresh potato Plastic wrap Refrigerator Salt, table, 2 grams Scissors Timer or clock Paper towels Cylinder-50-mL - Graduated, Plastic Dissection-kit with 7-tools - including the following: Bent Probe, Dropping Pipet, 2
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Probe, Ruler in pocket, Scalpel with 2 Blades - Note blades are in the pocket, Scissors, Tweezers Gloves, Disposable (1 pair) Goggles-Safety Funnel IKI Indicator, 2.1% - 7 mL in Glass Vial in Bubble Bag Rubber Bands Digital scale Test Tube, 13 x 100 mm in Bubble Bag Test-tube-rack-6x13-mm Test-tube-clamp-holder Dialysis Tubing - inches (6) Assembly Pencil, marking Pipet, Graduated Jumbo (5 mL) Test-tube-cleaning-brush Thermometer Rod, Stirring rod Benedict’s Reagent Glucose Solution in a Dropper Bottle Pipet, Empty Long Stem IKI Indicator, 7 mL, 2.1% Potassium Permanganate Crystals Starch Solution, 1% Stabilized Methods: Exercise 1- 1. Label two cups 1 & 2. 2. Use the graduated cylinder to measure and pour 150 mL of distilled water into cup 1. 3. Soak the dialysis tubing in the distilled water in cup 1 for 5 minutes.
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