277Dec-2000 - THE UNIVERSITY OF MANITOBA DATE: Dec. 19,...

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THE UNIVERSITY OF MANITOBA DATE: Dec. 19, 2000 Final EXAMINATION PAPER NO.: 554/555 PAGE NO.: 1 of 12 DEPARTMENT & COURSE NO.: 2.277/60.277 Time: 2 HOURS EXAMINATION: Elements of Biochemistry I EXAMINER: Drs. D.Burton and A. Scoot continued on next page. .. GENERAL INSTRUCTIONS 1. You must mark the answer sheet with pencil (not pen). 2. Put your name and enter your student number on the answer sheet. 3. The examination consists of multiple choice questions. Choose what you think is the best, correct answer and record your choice on the answer sheet. There is only ONE CORRECT answer. 4. This exam will count for 60% of your final mark. 2. Hydrophobic interactions: A) primarily involve the effect of polar solutes on the entropy of aqueous systems. B) do not contribute to the structure of water-soluble proteins. C) refer to the ability of water to denature proteins. D) are the driving force in the formation of micelles of amphipathic compounds in water 2. The pH of a sample of blood is 7.4. The pH of a sample of gastric juice is 1.4. The blood sample has: A) 5.29 times lower [H ] than the gastric juice. + B) 6 times lower [H ] than the gastric juice. + C) 6,000 times lower [H ] than the gastric juice. + D) a million times lower [H ] than the gastric juice. + E) 0.189 times the [H ] as the gastric juice. + a 3. Phosphoric acid is tribasic, with pK values of 2.14, 6.86 and 12.4. The ionic form that predominates at pH 3.2 is: 34 A) H PO 24 B) H PO 6 4 C) HPO 2 6 4 D) PO 3 6 a 4. A compound has a pK of 7.4. To 100 mL of a 1.0 M solution of this compound at pH 8.0 is added 30 mL of 1.0 M hydrochloric acid. The resulting solution is pH: A) 7.58 B) 7.4 C) 7.22 D) 6.8 E) 6.53 a 5. One hundred mL of 0.1 M NaOH is added to 55 mL of 0.2 M lactic acid. (The pK of lactic acid is 4.1). The resulting mixture has a pH close to: A) 2 B) 3 C) 4 D) 5
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THE UNIVERSITY OF MANITOBA DATE: Dec. 19, 2000 Final EXAMINATION PAPER NO.: 554/555 PAGE NO.: 2 of 12 DEPARTMENT & COURSE NO.: 2.277/60.277 Time: 2 HOURS EXAMINATION: Elements of Biochemistry I EXAMINER: Drs. D.Burton and A. Scoot continued on next page. .. E) 6 6. In a highly acidic solution, pH = 1, the dominant form of glycine is: 2 2 A) NH - CH - COOH 32 B) NH - CH - COOH + 2 2 C) NH - CH - COO & D) NH - CH - COO + & 2 3 E) NH - CH - COO + & 7. In an " -helix, the R groups on the amino acid residues: A) are found on the outside of the helix spiral. B) generate the hydrogen bonds that form the helix. C) stack within the interior of the helix. D) cause only right-handed helices to form. E) alternate between the outside and the inside of the helix. 8. Which of the following statements about proteins is true? A) Proteins are generally very loosely structured. B) In water-soluble proteins, hydrophobic (nonpolar) amino acid residues are generally buried and not exposed to water. C) Proteins that contain " -helical regions never contain $ sheets. D) “Corners” between " -helical regions in globular proteins invariably contain a glycine residue, which, because of its unique properties, cannot fit into the helix.
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277Dec-2000 - THE UNIVERSITY OF MANITOBA DATE: Dec. 19,...

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