Lab Quiz 4 notes.docx - Viruses Obligate intracellular...

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Viruses Obligate intracellular parasites Only reproduce within actively growing cells Subvert host to make new virus particles T4 Bacteriophage Infects E. coli o Attaches to E. coli and injects viral DNA o New virus particles are made o Cell lyses and spews out viral particles Lytic vs Lysogenic Lytic o Viral DNA injected into host cell o Forms new viral particles o Cells lyse and spew out viral particle content Lysogenic o Viral DNA injected and incorporated into host DNA o DNA transferred to daughter cell o Viral replication is delayed Virus Enumeration Viruses are 1/100 size of a bacterium Count with E.M. Viral plaque assay for lytic viruses Viral plaque assay: Method Viruses in dilution are mixed with bacteria and incubated Dilution is plated Plaques counted as PFU’s o PFUs are the number of virus particles Multiply # by dilution factor to get PFU/mL Yogurt production Milk steamed before inoculation to reduce oxygen content o Lactobacillus and Streptococcus spp. prefer low oxygen
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