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dilplat - Chris Gumulya Bio 126M Unique 51870 Dilution...

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Chris Gumulya Bio 126M Unique # 51870 Dilution Plating Lab 1) Purpose: The purpose of this lab was to learn about, and experiment with dilution plating. We learned how to calculate the concentration for each dilution step, how to calculate original titer, and thus the titer for each dilution step. We also learned about colorimetry and turbidimetry and how both were important methods for quantitative determination of concentrations. a) Procedure: The procedure consisted of two different experiments. i) The first purpose that we wanted to look at was dilution plating. To do this we first had a mixture of safranin and water. We diluted the original dye concentration from 100 to .1 in two different ways, first the practice dilution, and secondly to show that there is more than just one way to get to a certain dilution. ii) The second experiment also dealt with dilution but this time it wasn’t for practice. We took an unknown cell culture (I) and made an initial concentration. We then diluted that concentration according to the steps given to us. We then made plates for 6
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