patbac - Chris Gumulya Bio 126M Unique # 51870 Pathogenic...

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Chris Gumulya Bio 126M Unique # 51870 Pathogenic Bacteria Lab 1) Purpose: The purpose of this lab was to find guess what kind of bacteria made up an unknown culture by conducting different test and various platings. The three types of culture plates we used were blood agar, MacConkey’s agar, and Tryptic Soy Agar. Some new tests we learned to use were the Klinger’s Iron Agar, phenylalanine deaminase, citrate utilization, and the decarboxylase test. Some old biochemical tests we used were the carbohydrate fermentation test, the indole test, the catalase test, and a test for mobility. Before we started the lab we learned what each test was for and how to conduct it. a) Procedure: The procedure consisted of one experiment over a period of 4 days. i) On day 1, I streaked my unknown culture on my CNA Blood Agar, MacConkey Agar, and TSA plates. ii) On the second day I observed the colony morphology and did a gram stain on all 3 plates. On my blood agar plate, I observed the hemolysis of the gram positive bacteria, and also conducted a
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This note was uploaded on 11/10/2008 for the course BIO 126L taught by Professor Saxena during the Fall '08 term at University of Texas.

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patbac - Chris Gumulya Bio 126M Unique # 51870 Pathogenic...

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