04 TRANSPIRATION 2007 - Transpiration LAB Phaseolus...

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Transpiration LAB   Phaseolus vulgaris   L. (common bean plant)
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Objectives Investigate the effect of low light and high light intensity on the  rate of transpiration from leaves. Investigate the effect of abscisic acid (ABA) on the rate of  transpiration from leaves. Make and view slides of plant root, stem, and leaf sections using  bright field and fluorescence microscopy. Examine epidermal peels under the microscope.
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PHOTOSYNTHESIS
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Plant cells formed at meristems (. . . Then develop into cell types. .  .tissues) Plant tissue types Dermal: covers outer surface, prevent water loss Ground: bulk of primary plant body  
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Plant Terms… Vascular tissue Xylem Phloem
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XYLEM Complex vascular tissue through which most of the water and minerals are conducted from the roots to other parts of the plant. Cells are dead.
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XYLEM ELEMENTS * Vessels : Elongated, cells placed end to end. Walls at end of vessel members are perforated or are completly missing. Found in most angiosperms.
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XYLEM ELEMENTS Tracheid : Elongated, thick- walled conducting cells of xylem, characterized by tapering ends and pitted walls without true perforations.
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LIGNIN Hard supporting material embedded in the cellulose matrix of plant cell walls. Makes up xylem inner layer of cell (secondary) walls Second most common plant polymer Autofluoresces blue under UV light
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Phloem Vascular tissue that  conducts sugars and other  organic molecules from the  leaf to the other parts of  the plant.
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Sieve tubes Cells of phloem that line up  connecting leaves, shoots, and  roots. Cells contain little cytoplasm  (which is continuous between  cells) and no nucleus. Food materials pass from one  element to another via sieve  plates  (perforations).
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Companion Cell A parenchyma (ground tissue type) cell that  controls metabolic activities of sieve elements.
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PARENCHYMA Plant  tissue  composed  of  spongy,  living,  thin- walled  randomly  arranged  cells  with  large  vacuoles.    Usually  photosynthetic  or  storage  tissue. Most common type of cell in pants
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A = Xylem    B = Phloem Composed of dead cells Cytoplasm of one cell joined to adjacent cells Carries water Carries sugars and organic molecules Wall fluoresce blue under UV light
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Root Morphology Epidermis  ( outer dermal tissue absorbs water and  minerals from soil) Cortex  (ground tissue of root, storage parenchyma) Endodermis  (compact cells with no space between  strip) Vascular Cylinder  (xylem & phloem)
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Jepson, M. (1966). Biological Drawings with Notes , Parts I & II. Norwich:John Murray ( Publishers) Ltd., pp.22-23.
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04 TRANSPIRATION 2007 - Transpiration LAB Phaseolus...

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