lab11-Biological Forensics

lab11-Biological Forensics - Lab 11 Biological Forensics...

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Lab 11 Biological Forensics TA: Xiangyu Shi
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Gel electrophoresis (p. 22-2) Separate DNA according to their size Use DNA marker (ladder) to determine the size of DNA Step 1: Casting Agarose gel (p. 22-2) Casting gel - lift and tighten end gates - secure comb in the notches near the end gate - pour the agarose gel (up to the notch) End gate After the agarose solidified - lower the end gates - place the whole tray in buffer chamber - cover gel with 1x TBE (running buffer) - remove the comb - add just enough buffer to cover the wells (don’t add too much, otherwise the gel will float) Well 2
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Objectives Evaluate evidence using the Presumptive Blood Test. Evaluate hair evidence using a comparative approach. Evaluate fingerprint evidence. Run a gel electrophoresis to generate a DNA fingerprint. Generate a table of RFLP fragment data. Explain the principles of electrophoresis with respect to restriction fragment on DNA Write a report to the investigating officer describing the results of the forensic investigation.
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How does forensic identification work? Any type of organism can be identified by examination of DNA sequences unique to that species. Identifying individuals within a species is less precise at this time, although when DNA sequencing technologies progress farther, direct comparison of very large DNA segments, and possibly even whole genomes, will become feasible and practical and will allow precise individual identification To identify individuals, forensic scientists scan 13 DNA regions that vary from person to person and use the data to create a DNA profile of that individual (sometimes called a DNA fingerprint). There is an extremely small chance that another person has the same DNA profile for a particular set of regions. Forensic evidence is any object or material that may connect an offender to a crime scene. Physical evidence : weapons, ballistics results, tire tracks, possession of stolen materials, or witness testimony Biological evidence : DNA, blood, fingerprint or hair analysis
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Some Examples of DNA Uses for Forensic Identification Identify potential suspects whose DNA may match evidence left at crime scenes Exonerate persons wrongly accused of crimes Identify crime and catastrophe victims Establish paternity and other family relationships Identify endangered and protected species as an aid to wildlife officials (could be used for prosecuting poachers) Detect bacteria and other organisms that may pollute air, water, soil, and food Match organ donors with recipients in transplant programs Determine pedigree for seed or livestock breeds
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Forensic Science: DNA Fingerprinting DNA testing can identify a guilty individual with a high degree of certainty because the DNA sequence of every person is unique (except for identical twins) There are several DNA fingerprinting tests
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What are some of the DNA technologies used in forensic investigations? Restriction Fragment Length Polymorphism (RFLP)
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lab11-Biological Forensics - Lab 11 Biological Forensics...

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