Lab 3 Biomolecules

Lab 3 Biomolecules - WELCOME TO BIOLOGY 111

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SAFETY ISSUES Bring SAFETY GOGGLES to EVERY LAB. If you do not have goggles, rent a pair from the Chemistry Department on the 4 th floor. No goggles = no working in the lab!!! No open-toed shoes! Beware of the electrical plug points!! Beware of toxic & hazardous chemicals!!! (Do not inhale or ingest even for fun anything chemical!!)
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BIOMOLECULES Lab #3
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Biomolecules/Organic Molecules Organic molecules are necessary for biological processes. Organic molecules contain carbon . 4 major types of organic molecules: Saccharides Lipids Proteins Nucleic Acids (We’ll look at these in a future lab.)
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Saccharides/Carbohydrates Organic molecules are classified by the presence of various functional groups . These functional groups determine how an organic molecule will react in a chemical reaction. Carbohydrates are characterized by the presence of a terminal aldehyde group or an internal ketone group.
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Saccharides Serve as a major nutrient and as a building material for cells. The base unit (monomer ) of carbohydrates is the monosaccharide (single sugar). Monosaccharides are classified by the number of carbon atoms they contain. Trioses Pentoses Hexoses Monosaccarides may exist as rings (stable) or linearly (unstable)
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Saccharides Monosaccharides link together via a dehydration synthesis reaction. Disaccharides Polysaccharides Starch (plants) Glycogen (animals) Monomers come together to form polymers .
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Saccharides Reducing sugars : contain a free aldehyde or keytone group Includes: All monosaccharides Disaccharides where one aldehyde or keytone group is free
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Benedict’s Test Tests for the presence of reducing sugars (reacts with the free aldehyde or ketone groups) You will fill in the data for Table 3-1. Procedure begins on pg. 29 You will need 8 test tubes to test the 8 solutions in Table 3-1. Make sure you gently shake the solution bottles before you use them. Use parafilm to mix your solutions with the Benedict’s reagent in the test tubes. WARNING! BENEDICT’S REAGENT IS A CORROSIVE BASE . DO NOT COME IN CONTACT WITH THIS CHEMICAL AND DISPOSE IN BASE WASTE CONTAINER.
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A positive test result is any color change. Color changes range
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Lab 3 Biomolecules - WELCOME TO BIOLOGY 111

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