October_21_lecture_2_slides_per_page - Transcription (but...

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1 DNA/RNA gels
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2 EcoRI EcoRI Wells (start) +
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3 RNA Synthesis : Transcription Chapter 29 of Berg, Tomoczko and Stryer and Chapter 11, Boyer - Prokaryotic transcription : promoter binding and initiation elongation termination - Eukaryotic transcription : distinctions b/w prokaryotes RNA polymerase
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4 RNA Polymerase binds to Promoter Site Promoter Region in Prokaryotes
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5 Consensus sequence of the TATA box Mechanism of Transcription
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6
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7
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8 Elongation:
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9 Termination of Transcription Inhibitors of Transcription Rifampycin - α -Amanitin - Actinomycin D -
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10 Modification of RNA after
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Unformatted text preview: Transcription (but not mRNA!!) Transcription in Eukaryotes 11 Common Elements (Cis-acting) in RNA Polymerase II Promoter Region 12 13 Enhancer sequences- Bind many other transcription factors (called enhancers)- Can be thousands base pairs away from promoter. Processing of mRNA- Products of RNA Polymerase II called pre-mRNA: (i) cap (ii) poly (A) (iii) “splicing” 14 3’ Poly(A) Tail pre-mRNA 15 Splicing of Exons/Introns 16 Splice sites of pre-mRNA Transesterification Reactions 17...
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October_21_lecture_2_slides_per_page - Transcription (but...

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