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HW 1 - Biological Sciences 102 Homework#1 Spring 2009 Prof...

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Biological Sciences 102 Spring, 2009 Homework #1 Prof R Holland Cheng Learn the structures and the codes of the twenty amino acids ( Table, pp. 78-79, Chapter 4, Garrett & Grisham) ** Use the following table of pK a ’s to answer HW#1 problems. Compound pK a ’s Phosphate 2.12, 7.21, 12.32 Tris 8.21 Amino acid side chains C 8.3 D 3.9 E 4.2 H 6.0 K 10.5 R 12.5 Y 10.1 Amino acid -carboxyl group 2.1 Amino acid -amino group 9.6 Oligopeptide N-terminal amino group 7.4 Oligopeptide C-terminal carboxyl group 3.6 1. Why is it important that weak forces, not strong forces, mediate biomolecular recognition interactions? Biomolecules interact with one another through molecular surfaces that are structurally complementary. How can various proteins interact with molecules as different as simple ions, hydrophobic lipids, polar but uncharged carbohydrates, and even nucleic acids? (Adapted from Garrett & Grisham, Biochemistry , 3 rd ed., Thomson Brooks/ Cole, c2005) 2. If the internal pH of a muscle cell is 6.8, what is the [HPO 4 ]/[H 2 PO 4 ] ratio in this cell? Why do we use the Henderson-Hasselbalch equation to solve this problem? Why do we not just subtract or add molar amounts of acid and
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