FinalStudy_Guide_for_Lab_Competency_ExamF05 - Study Guide...

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Study Guide for Lab Competency Exam, Fall 2005 Lab Competency Exam (LCE) Week of November 28 50 point (~5 points/station) A. every student should have signed up for LCE B. Your lab instructor can add to this study guide as much as he or she wants C. student miss with excuse should call Tom to do make up, immediately D. time each station for 4 minutes; except the graphing which is 8 minutes E. there is no extra time at end; so make sure you look over your exam when you get done a station early E. sign up is because we have to split class and cannot have more than 12 students/time (ideal is 10 students) F. there are 10 - 12 stations in the room which everyone must complete (if your lab instructor wants to add to this, he or she can) G. Once you leave a station you cannot go back, so if you needed a letter for an answer you cannot look back By the end of the semester, you should also be able to: 1. Perform all basic techniques (measuring, balancing, streaking etc.) learned in this lab and subsequent labs. 2. Identify each tool, safety item and piece of equipment used in this lab. 3. Based on new questions, be able to design controlled experiments using the tools, techniques and equipment used in this course. 4. When given data for an experiment, determine whether the data is accurate or not based on how the technique is performed (e.g., isolation streaking technique did not yield isolated colonies but only had a lawn of growth—someone did not perform the correct technique or the student did not sterilize the loop between zones; the centrifuge is not balanced; spectrophotometer data that had not been adjusted (zeroed) with the blank so you cannot create a standard curve, etc.) 5. Complete the following tasks for these 10 stations. 6. Describe what is the purpose for using the following different pieces of equipment: centrifuge, microscope, spectrophotometer and gel electrophoresis box. Plating and Transformation Techniques Station (1) 1. Use the aseptic protocol and the proper tools, to perform the isolation (3-zone) streaking technique to yield isolated colonies in zone 3. Also, be prepared to identify which tools are used for this technique. 2. Use the aseptic protocol and the proper tools, to perform the confluency streaking technique to yield a lawn of growth. Also, be prepared to identify which tools are used for this technique. 3. Be prepared to demonstrate your aseptic technique and streaking to your lab instructor. 4. Describe or draw how a plate is streaked for the confluency streaking technique or the isolation streaking technique. 5. List the materials needed for the confluency streaking technique and isolation streaking technique. 6. When given a plate with bacterial growth, be able to identify which streaking technique was used. 7.
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This note was uploaded on 03/19/2008 for the course BS 111L taught by Professor Alison during the Spring '08 term at Michigan State University.

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FinalStudy_Guide_for_Lab_Competency_ExamF05 - Study Guide...

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